Primeiro registro de Rachiplusia nu (Guenée, 1852) (Lepidoptera: Noctuidae) sobrevivendo em soja Bt no Brasil

Adriana C. Nardon; Sandra M. Mathioni; Leandro V. dos Santos; Daniel D. Rosa

doi:10.37486/2675-1305.ec03028

Authors

Adriana C. Nardon Syngenta Proteção de Cultivos, São Paulo, São Paulo https://orcid.org/0000-0003-2157-1622
Sandra M. Mathioni Syngenta Proteção de Cultivos, São Paulo, São Paulo https://orcid.org/0000-0002-8853-4108
Leandro V. dos Santos Syngenta Proteção de Cultivos, São Paulo, São Paulo https://orcid.org/0000-0003-4856-2144
Daniel D. Rosa Syngenta Proteção de Cultivos, São Paulo, São Paulo https://orcid.org/0000-0003-4996-417X

DOI:

https://doi.org/10.37486/2675-1305.ec03028

Keywords:

Soybean, soybean looper, insect, pest, sunflower looper

Abstract

Loopers are important defoliators in several crop species. Among the loopers attacking non-Bt soybean cultivars, Chrysodeixis includens (Walker, [1858]) was the most common. The use of trangenic Bt soybean in appromately 70% of the soybean fields in Brazil led to a decrease in the loopers in these fields. However, technical field specialists and soybean producers recently observed Plusiinae larvae damaging transgenic Bt soybean, posing a risk over the Bt technology. Therefore, the aim of this study was to identify and confirm the catterpillar species feeding on transgenic Bt soybean fields in Brazil. Larvae and soybean leaf samples were collected in 12 soybean-producing locations from four Brazillian States. Soybean leaf samples were tested to confirm whether they were transgenic Bt. Larvae were reared in artificial diet and submitted to feeding trials on non-transgenic and transgenic Bt soybean leaves. The species identification was perfomed by amplicon sequencing of the cytochrome oxidase I (COI) gene of eight larvae per local. All 12 populations fed in the transgenic Bt soybean and the sequencing analysis of COI determined that they belong to Rachiplusia nu (Guenée, 1852) with 100% similarity to the COI sequences available in NCBI for this species. These results suggest that the R. nu species is not controlled by the genetic modification event present in the tested soybean variety and highlights the need for additional looper management control strategies, such as insecticide application, for controlling this insect pest in transgenic Bt soybean.

Downloads

Download data is not yet available.

References

Folmer, O.; Black, M.; Hoeh, W.; Lutz, R.; Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294-299.

Greene, G. L.; Leppla, N. C.; Dickerson, W. A. (1976) Velvetbean caterpillar: a rearing procedure and artificial medium. Journal of Economic Entomology, 69(4): 447-448.

Moraes, R. R.; Loeck, A. E.; Belarmino, L. C. (1991) Inimigos Naturais de Rachiplusia nu (GUENÉE, 1852) e de Pseudoplusia includens (WALKER, 1857) (LEPIDOPTERA: NOCTUIDAE) em Soja no Rio Grande do Sul. Pesquisa Agropecuária Brasileira, 26(1): 57-64.

Moscardi, F.; Bueno, A. F.; Sosa-Gómez, D. R.; Roggia, S.; Hoffmann-Campo, C. B.; Pomari, A. F.; Corso, I. C.; Yano, S. A. C. (2012) Artrópodes que atacam as folhas da soja. In: Hoffmann-Campo, C. B.; Corrêa-Ferreira, B. S.; Moscardi, F. (Eds.), Soja: manejo integrado de insetos e outros artrópodes-praga, pp. 213-334. Londrina: Embrapa.

Perini, C. R.; Stacke, R. F.; Stacke, R. S.; Froehlich, R.; Machado, D. N.; Puntel, V. L.; Ceolin, M.; Daltrozo, W. B.; Guedes, J. C. (2020) Lacy horizon. Revista Cultivar. https://www.grupocultivar.com.br/ativemanager/uploads/arquivos/artigos/bab64942e191a19b96e1e84a1c4aeb97.pdf. Acessado em: 9.vi.2021.

Perini, C. R.; Sosa, V. I.; Koda, V. E.; Silva, H.; Risso, A. A.; Vasconcelos, W. N. F.; Gonçalves, C. F.; Ugalde, G. A.; Machado, D. N.; Bevilacqua, C. B.; Ardisson-Araújo, D. M.; Maebe, K.; Smagghe, G.; Valmorbida, I.; Guedes, J. C. (2021) Genetic structure of two Plusiinae species suggests recent expansion of Chrysodeixis includens in the American continent. Agricultural and Forest Entomology, 23: 250-260. doi: 10.1111/afe.12427

Pozebon, H.; Marques, R. P.; Padilha, G.; O’Neal, M.; Valmorbida, I.; Bevilaqua, J. G.; Tay, W. T.; Arnemann, J. A. (2020) Arthropod invasions versus soybean production in Brazil: a review. Journal of Economic Entomology, 113(4): 1591-1608. doi: 10.1093/jee/toaa108

Simon, C.; Frati, F.; Beckenbach, A.; Crespi, B.; Liu, H.; Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87: 651-701.

Sosa-Gómez, D. R.; Corrêa-Ferreira, B. S.; Hoffmann-Campo, C. B.; Corso, I. C.; Oliveira, L. J.; Moscardi, F.; Panizzi, A. R.; Bueno, A. F.; Hirose, E. (2010) Manual de identificação de insetos e outros invertebrados da cultura da soja. Londrina: Embrapa Soja.

Thompson, J. D.; Higgins, D. G.; Gibson, T. J. (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-4680.